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Characterization of a laccase from a wood-feeding termite, Coptotermes formosanus

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Purification and characterization of a novelanti-coagulant from the leech Hirudinaria manillensis

【摘要】Protease inhibitors have been reported rarely fromthe leech Hirudinaria manillensis. In this study, wepurified a novel protease inhibitor (bdellin-HM-2) withanticoagulant properties from H. manillensis. With amolecular weight of 1.4x104, bdellin-HM-2 was alsocharacterized with three intra-molecular disulfidebridges at the N-terminus and multiple HHXDD andHXDDmotifs at the C-terminus. cDNAcloning revealedthat the putative nucleotide-encoding protein ofbdellin-HM-2 contained 132 amino acids and wasencoded by a 399 bp open reading frame (ORF)。 Sequence alignment showed that bdellin-HM-2 sharedsimilarity with the“non-classical”Kazal-type serineprotease inhibitors, but had no inhibitory effect ontrypsin, elastase, chymotrypsin, kallikrein, factor XIIa(FXIIa), factor XIa (FXIa), factor Xa (FXa), thrombin,or plasmin. Bdellin-HM-2 showed anticoagulant effectsby prolonging the activated partial thromboplastin time(aPTT), indicating a role in enabling H. manillensis toobtain a blood meal from its host. Our resultssuggest that bdellin-HM-2 may play a crucial role inblood-sucking in this leech species and may be apotential candidate for the development of clinicalanti-thrombotic drugs.

《动物学研究》 | 2019年3期 | Ruo-Mei Cheng

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《中国纺织(英文版)》 | 2019年1期 | Irene Muggler

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A Spectroscopic Study on Tautomerism of Selaginellins from Selaginella

【摘要】Ultraviolet-visible and nuclear magnetic resonance (NMR) spectroscopy was used to investigate the effects of pH, solvent and temperature on the tautomeric equilibrium of selaginellins from Selaginella. The results demonstrated that the tautomerism of selaginellins could be catalyzed by acid, base and protic solvent. Variable-temperature 1HNMRexperiments demonstrated that the tautomerism was interrupted when the temperature fell below 233 K.

《波谱学杂志》 | 2019年2期 | CAOYuan

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Development and characterization of a guinea pig model for Marburg virus

【摘要】The Angolan strain of Marburg virus (MARV/Ang) can cause lethal disease in humans with a case fatality rate of up to 90%, but infection of immunocompetent rodents do not result in any observable symptoms. Our previous work includes the development and characterization of a MARV/Ang variant that can cause lethal disease in mice (MARV/Ang-MA), with the aim of using this tool to screen for promising prophylactic and therapeutic candidates. An intermediate animal model is needed to confirm any findings from mice studies before testing in the gold-standard non-human primate (NHP) model. In this study, we serially passaged the clinical isolate of MARV/Ang in the livers and spleens of guinea pigs until a variant emerged that causes 100% lethality in guinea pigs (MARV/Ang-GA)。 Animals infected with MARV/Ang-GA showed signs of filovirus infection including lymphocytopenia, thrombocytopenia, and high viremia leading to spread to major organs, including the liver, spleen, lungs, and kidneys. The MARV/Ang-GA guinea pigs died between 7-9 days after infection, and the LD50 was calculated to be 1.1×10-1 TCID50 (median tissue culture infective dose)。 Mutations in MARV/Ang-GA were identified and compared to sequences of known rodent-adapted MARV/Ang variants, which may benefit future studies characterizing important host adaptation sites in the MARV/Ang viral genome.

《动物学研究》 | 2018年1期 | Gary Wong

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Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis

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A new species of smooth skink (Squamata: Scincidae: Scincella) from Cambodia

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《动物学研究》 | 2018年3期 | Thy Neang

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《动物学研究》 | 2019年6期 | Yun-He Wu

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