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Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

【摘要】热吃惊蛋白质(Hsps)在昆虫被连接了到压力和冬季diapause,但是他们是否是夏天diapause的部件,仍然是未知的。分别地,在这研究,从Pierismelete的Hsp90的互补DNA,Pierisrapae和Pieriscanidia,命名PmHsp90,PrHsp90和PcHsp90被克隆并且定序。推出的氨基酸顺序分别地与82.6,82.6和82.7kDa的一个通常认为的分子的团由718氨基酸残余组成了。氨基酸序列在Hsp90家庭和合拢活动区域的bHLH蛋白质五个保存签名主题包含了所有。响应夏天diapause和冬季diapause的PmHsp90的微分表示模式,与热/寒冷应力有关,被调查。冷应力在夏天和冬季diapause蛹,然而并非在nondiapause个人导致了Hsp90upregulation。热吃惊upregulatedPmHsp90逐渐地随在夏天diapause,和PmHsp90的温度的增加是很快在冬季diapause的upregulated。在39点在30min热以后震惊牥捡慥????????????????吗??

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